The transmembrane adaptor protein NTAL limits mast cell chemotaxis toward prostaglandin E-2

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F18%3A00497556" target="_blank" >RIV/68378050:_____/18:00497556 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1126/scisignal.aao4354" target="_blank" >http://dx.doi.org/10.1126/scisignal.aao4354</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1126/scisignal.aao4354" target="_blank" >10.1126/scisignal.aao4354</a>

Result language
angličtina
Original language name
The transmembrane adaptor protein NTAL limits mast cell chemotaxis toward prostaglandin E-2
Original language description
Chemotaxis of mast cells is one of the crucial steps in their development and function. Non-T cell activation linker (NTAL) is a transmembrane adaptor protein that inhibits the activation of mast cells and B cells in a phosphorylation-dependent manner. Here, we studied the role of NTAL in the migration of mouse mast cells stimulated by prostaglandin E-2 (PGE(2)). Although PGE(2) does not induce the tyrosine phosphorylation of NTAL, unlike IgE immune complex antigens, we found that loss of NTAL increased the chemotaxis of mast cells toward PGE(2). Stimulation of mast cells that lacked NTAL with PGE(2) enhanced the phosphorylation of AKT and the production of phosphatidylinositol 3,4,5-trisphosphate. In resting NTAL-deficient mast cells, phosphorylation of an inhibitory threonine in ERM family proteins accompanied increased activation of beta 1-containing integrins, which are features often associated with increased invasiveness in tumors. Rescue experiments indicated that only full-length, wild-type NTAL restored the chemotaxis of NTAL-deficient cells toward PGE(2). Together, these data suggest that NTAL is a key inhibitor of mast cell chemotaxis toward PGE(2), which may act through the RHOA/ERM/beta 1-integrin and PI3K/AKT axes.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30202 - Endocrinology and metabolism (including diabetes, hormones)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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