Expression of the chicken GDNF family receptor alpha-1 as a marker of spermatogonial stem cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F13%3A00422220" target="_blank" >RIV/68378050:_____/13:00422220 - isvavai.cz</a>
Alternative codes found
RIV/46356606:_____/13:#0000042
Result on the web
<a href="http://dx.doi.org/10.1016/j.anireprosci.2013.08.006" target="_blank" >http://dx.doi.org/10.1016/j.anireprosci.2013.08.006</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.anireprosci.2013.08.006" target="_blank" >10.1016/j.anireprosci.2013.08.006</a>

Result language
angličtina
Original language name
Expression of the chicken GDNF family receptor alpha-1 as a marker of spermatogonial stem cells
Original language description
The identification, enrichment and subsequent isolation of spermatogonial stem cells (SSCs) are integral to the success of SCC transplants between fertile donor and sterilized recipient males. In birds generally and particularly in chicken, SSC-specifichas yet to be identified. The receptor for glial cell-derived neurotrophic factor (GDNF), i.e. GDNF family receptor alpha-1 (GFR alpha 1), has been identified as a potential marker for different mouse spermatogonial subtypes. In the present study, we characterized the chicken cGFR alpha 1 receptor and compared its predicted amino-acid sequence with mouse, rat and human GFR alpha 1 proteins. Using specific polyclonal mouse anti-cGFR alpha 1 serum, a total of 2.8% cells were recognized as cGFR alpha 1 positive among isolated testicular cells recovered from sexually mature cockerels. The percentages of cGFR alpha 1-positive testicular cells with haploid, diploid, tetraploid and SP DNA content were 1.6%, 2.5%, 393% and 76.8%, respectively.
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)