Role of DNA methylation in expression and transmission of porcine endogenous retroviruses

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F13%3A00422229" target="_blank" >RIV/68378050:_____/13:00422229 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1128/JVI.03262-12" target="_blank" >http://dx.doi.org/10.1128/JVI.03262-12</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/JVI.03262-12" target="_blank" >10.1128/JVI.03262-12</a>

Result language

angličtina

Original language name

Role of DNA methylation in expression and transmission of porcine endogenous retroviruses

Original language description

Porcine endogenous retroviruses (PERV) represent a major safety concern in pig-to-human xenotransplantation. To date, no PERV infection of a xenograft recipient has been recorded; however, PERVs are transmissible to human cells in vitro. Some recombinants of the A and C PERV subgroups are particularly efficient in infection and replication in human cells. Transcription of PERVs has been described in most pig cells, but their sequence and insertion polymorphism in the pig genome impede identification oftranscriptionally active or silenced proviral copies. Furthermore, little is known about the epigenetic regulation of PERV transcription. Here, we report on the transcriptional suppression of PERV by DNA methylation in vitro and describe heavy methylation in the majority of PERV 5' long terminal repeats (LTR) in porcine tissues. In contrast, we have detected sparsely methylated or nonmethylated proviruses in the porcine PK15 cells, which express human cell-tropic PERVs. We also demonstra

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/LK11215" target="_blank" >LK11215: Regulation of active endogenous retroviruses in the mammalian genome</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Virology

ISSN

0022-538X

e-ISSN

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Volume of the periodical

87

Issue of the periodical within the volume

22

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

12110-12120

UT code for WoS article

000325865400013

EID of the result in the Scopus database

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