Stabilization of protein by freeze-drying in the presence of trehalose: a case study of tubulin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F14%3A00434353" target="_blank" >RIV/68378050:_____/14:00434353 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-62703-977-2_32" target="_blank" >http://dx.doi.org/10.1007/978-1-62703-977-2_32</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-62703-977-2_32" target="_blank" >10.1007/978-1-62703-977-2_32</a>
Alternative languages
Result language
angličtina
Original language name
Stabilization of protein by freeze-drying in the presence of trehalose: a case study of tubulin
Original language description
Microtubules, polymers of the heterodimeric protein ??-tubulin, are indispensable for many cellular activities such as maintenance of cell shape, division, migration, and ordered vesicle transport. In vitro assays to study microtubule functions and theirregulation by associated proteins require the availability of assembly-competent purified tubulin. However, tubulin is a thermolabile protein that rapidly converts into non-polymerizing state. For this reason it is usually stored at 80 °C to preserve its conformation and polymerization properties. In this chapter we describe a method for freeze-drying of assembly-competent tubulin in the presence of nonreducing sugar trehalose and methods enabling evaluation of tubulin functions in rehydrated samples.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Methods in Molecular Biology
ISSN
1064-3745
e-ISSN
—
Volume of the periodical
1129
Issue of the periodical within the volume
February
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
443-458
UT code for WoS article
000337104300033
EID of the result in the Scopus database
—