Stabilization of proteins by freeze-drying in the presence of trehalose: a case study of tubulin

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F21%3A00540021" target="_blank" >RIV/68378050:_____/21:00540021 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/protocol/10.1007%2F978-1-0716-0775-6_27" target="_blank" >https://link.springer.com/protocol/10.1007%2F978-1-0716-0775-6_27</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-0775-6_27" target="_blank" >10.1007/978-1-0716-0775-6_27</a>

Result language
angličtina
Original language name
Stabilization of proteins by freeze-drying in the presence of trehalose: a case study of tubulin
Original language description
Microtubules, polymers of the heterodimeric protein αβ-tubulin, are indispensable for many cellularnactivities such as maintenance of cell shape, division, migration, and ordered vesicle transport. In vitronassays to study microtubule functions and their regulation by associated proteins require the availability of assembly-competent purified tubulin. However, tubulin is a thermolabile protein that rapidly converts into a nonpolymerizing state. For this reason, it is usually stored at -80 C or liquid nitrogen to preserve its conformation and polymerization properties. In this chapter, we describe a method for freeze-drying of assembly-competent tubulin in the presence of nonreducing sugar trehalose, and methods enabling the evaluation of tubulin functions in rehydrated samples.n
Czech name
—
Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name
Protein downstream processing: Design, Development And Application of High and Low-Resolution Methods (N. Labrou Ed.) 2nd edition
ISBN
978-1-0716-0775-6
Number of pages of the result
19
Pages from-to
417-435
Number of pages of the book
511
Publisher name
Humana Press
Place of publication
New York
UT code for WoS chapter
000697782600028

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