The first mouse mutants of D14Abb1e (Fam208a) show that it is critical for early development

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F14%3A00435131" target="_blank" >RIV/68378050:_____/14:00435131 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00335-014-9516-0" target="_blank" >http://dx.doi.org/10.1007/s00335-014-9516-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00335-014-9516-0" target="_blank" >10.1007/s00335-014-9516-0</a>

Result language

angličtina

Original language name

The first mouse mutants of D14Abb1e (Fam208a) show that it is critical for early development

Original language description

An ENU mutagenesis screen to identify novel epigenetic modifiers was established in mice carrying a multi-copy GFP transgene, which is expressed in a variegated manner in erythrocytes and is highly sensitive to epigenetic silencing. The screen has produced mouse mutants of both known modifiers of epigenetic state, such as Dnmt1 and Smarca5, and novel modifiers, such as Smchd1 and Rlf. Here we report two mouse lines generated from the screen, MommeD6 and MommeD20, with point mutations in D14Abb1e. Theseare the first mouse mutants of D14Abb1e (also known as Fam208a), a gene about which little is known. Heterozygous intercrosses show that homozygous mutants from both the MommeD6 and MommeD20 lines are not viable beyond gastrulation, demonstrating an important role for D14Abb1e in development. We demonstrate that haploinsufficiency for D14Abb1e effects transgene expression at the RNA level. Analysis of the predicted D14Abb1e protein sequence reveals that it contains putative nuclear local

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Mammalian Genome

ISSN

0938-8990

e-ISSN

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Volume of the periodical

25

Issue of the periodical within the volume

7-8

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

293-303

UT code for WoS article

000339875100002

EID of the result in the Scopus database

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