L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F14%3A00436786" target="_blank" >RIV/68378050:_____/14:00436786 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1189/jlb.2A1013-564R" target="_blank" >http://dx.doi.org/10.1189/jlb.2A1013-564R</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1189/jlb.2A1013-564R" target="_blank" >10.1189/jlb.2A1013-564R</a>

Result language
angličtina
Original language name
L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration
Original language description
Membrane rafts are microdomains of the plasma membrane that have multiple biological functions. The involvement of these structures in the biology of T cells, namely in signal transduction by the TCR, has been widely studied. However, the role of membrane rafts in immunoreceptor signaling in NK cells is less well known. We studied the distribution of the activating NKG2D receptor in lipid rafts by isolating DRMs in a sucrose density gradient or by raft fractionation by ?-OG-selective solubility in the NKL cell line. We found that the NKG2D-DAP10 complex and pVav are recruited into rafts upon receptor stimulation. Qualitative proteomic analysis of these fractions showed that the actin cytoskeleton is involved in this process. In particular, we found that the actin-bundling protein L-plastin plays an important role in the clustering of NKG2D into lipid rafts. Moreover, coengagement of the inhibitory receptor NKG2A partially disrupted NKG2D recruitment into rafts. Furthermore, we demonstr
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/GBP302%2F12%2FG101" target="_blank" >GBP302/12/G101: Molecular mechanisms of signaling through leukocyte receptors their role in health and disease.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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