Stochastic NANOG fluctuations allow mouse embryonic stem cells to explore pluripotency

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F14%3A00442504" target="_blank" >RIV/68378050:_____/14:00442504 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1242/dev.108910" target="_blank" >http://dx.doi.org/10.1242/dev.108910</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/dev.108910" target="_blank" >10.1242/dev.108910</a>

Result language

angličtina

Original language name

Stochastic NANOG fluctuations allow mouse embryonic stem cells to explore pluripotency

Original language description

Heterogeneous expression of the transcription factor NANOG has been linked to the existence of various functional states in pluripotent stem cells. This heterogeneity seems to arise from fluctuations of Nanog expression in individual cells, but a thorough characterization of these fluctuations and their impact on the pluripotent state is still lacking. Here, we have used a novel fluorescent reporter to investigate the temporal dynamics of NANOG expression in mouse embryonic stem cells (mESCs), and to dissect the lineage potential of mESCs at different NANOG states. Our results show that stochastic NANOG fluctuations are widespread in mESCs, with essentially all expressing cells showing fluctuations in NANOG levels, even when cultured in ground-state conditions (2i media). We further show that fluctuations have similar kinetics when mESCs are cultured in standard conditions (serum plus leukemia inhibitory factor) or ground-state conditions, implying that NANOG fluctuations are inherent

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GBP305%2F12%2FG034" target="_blank" >GBP305/12/G034: Centre for RNA Biology</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Development

ISSN

0950-1991

e-ISSN

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Volume of the periodical

141

Issue of the periodical within the volume

14

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

2770-2779

UT code for WoS article

000341303500004

EID of the result in the Scopus database

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