RecQ-core of BLM unfolds telomeric G-quadruplex in the absence of ATP

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F14%3A00455781" target="_blank" >RIV/68378050:_____/14:00455781 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1093/nar/gku856" target="_blank" >http://dx.doi.org/10.1093/nar/gku856</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gku856" target="_blank" >10.1093/nar/gku856</a>

Result language

angličtina

Original language name

RecQ-core of BLM unfolds telomeric G-quadruplex in the absence of ATP

Original language description

Various helicases and single-stranded DNA (ss-DNA) binding proteins are known to destabilize G-quadruplex (GQ) structures, which otherwise result in genomic instability. Bulk biochemical studies have shown that Bloom helicase (BLM) unfolds both intermolecular and intramolecular GQ in the presence of ATP. Using single molecule FRET, we show that binding of RecQ-core of BLM (will be referred to as BLM) to ssDNA in the vicinity of an intramolecular GQ leads to destabilization and unfolding of the GQ in theabsence of ATP. We show that the efficiency of BLM-mediated GQ unfolding correlates with the binding stability of BLM to ssDNA overhang, as modulated by the nucleotide state, ionic conditions, overhang length and overhang directionality. In particular,we observed enhanced GQ unfolding by BLM in the presence of non-hydrolysable ATP analogs, which has implications for the underlying mechanism. We also show that increasing GQ stability, via shorter loops or higher ionic strength, reduces

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA204%2F09%2F0565" target="_blank" >GA204/09/0565: Role of RECQ5 DNA helicase in maintenance of genomic stability</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

42

Issue of the periodical within the volume

18

Country of publishing house

GB - UNITED KINGDOM

Number of pages

18

Pages from-to

11528-11545

UT code for WoS article

000347687100029

EID of the result in the Scopus database

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