The human RecQ helicases BLM and RECQL4 cooperate to preserve genome stability

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F12%3A00390249" target="_blank" >RIV/68378050:_____/12:00390249 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1093/nar/gks349" target="_blank" >http://dx.doi.org/10.1093/nar/gks349</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gks349" target="_blank" >10.1093/nar/gks349</a>

Result language
angličtina
Original language name
The human RecQ helicases BLM and RECQL4 cooperate to preserve genome stability
Original language description
Bacteria and yeast possess one RecQ helicase homolog whereas humans contain five RecQ helicases, all of which are important in preserving genome stability. Three of these, BLM, WRN and RECQL4, are mutated in human diseases manifesting in premature agingand cancer. We are interested in determining to which extent these RecQ helicases function cooperatively. Here, we report a novel physical and functional interaction between BLM and RECQL4. Both BLM and RECQL4 interact in vivo and in vitro. We have mapped the BLM interacting site to the N-terminus of RECQL4, comprising amino acids 361-478, and the region of BLM encompassing amino acids 1-902 interacts with RECQL4. RECQL4 specifically stimulates BLM helicase activity on DNA fork substrates in vitro. Thein vivo interaction between RECQL4 and BLM is enhanced during the S-phase of the cell cycle, and after treatment with ionizing radiation. The retention of RECQL4 at DNA double-strand breaks is shortened in BLM-deficient cells. Further, de
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/GAP305%2F10%2F0281" target="_blank" >GAP305/10/0281: Úloha produktu genu Rothmund-Thomsonova syndromu při údržbě genetické stability</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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