DNA2 cooperates with the WRN and BLM RecQ helicases to mediate long-range DNA end resection in human cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F14%3A00436394" target="_blank" >RIV/68378050:_____/14:00436394 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1074/jbc.M114.578823" target="_blank" >http://dx.doi.org/10.1074/jbc.M114.578823</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1074/jbc.M114.578823" target="_blank" >10.1074/jbc.M114.578823</a>

Result language
angličtina
Original language name
DNA2 cooperates with the WRN and BLM RecQ helicases to mediate long-range DNA end resection in human cells
Original language description
The 5'-3' resection of DNA ends is a prerequisite for the repair of DNA double strand breaks by homologous recombination, microhomology-mediated end joining, and single strand annealing. Recent studies in yeast have shown that, following initial DNA endprocessing by the Mre11-Rad50-Xrs2 complex and Sae2, the extension of resection tracts is mediated either by exonuclease 1 or by combined activities of the RecQ family DNA helicase Sgs1 and the helicase/endonuclease Dna2. Although human DNA2 has been shown to cooperate with the BLM helicase to catalyze the resection of DNA ends, it remains a matter of debate whether another human RecQ helicase, WRN, can substitute for BLM in DNA2-catalyzed resection. Here we present evidence that WRN and BLM act epistatically with DNA2 to promote the long-range resection of double strand break ends in human cells. Our biochemical experiments show that WRN and DNA2 interact physically and coordinate their enzymatic activities to mediate 5'-3' DNA end res
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/GAP305%2F10%2F0281" target="_blank" >GAP305/10/0281: Úloha produktu genu Rothmund-Thomsonova syndromu při údržbě genetické stability</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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