BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F09%3A00333639" target="_blank" >RIV/68378050:_____/09:00333639 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation
Original language description
Bloom syndrome (BS) is characterized by genomic instability and high predisposition to cancer. BLM, gene defective in BS, encodes a member of the RecQ family of 3´-5´ DNA helicases and is proposed to function in recombinational repair at DNA replication.We used single-molecule fluorescence resonance energy transfer microscopy to examine BLM behaviour on forked DNA substrates. BLM unwound individual DNA molecules repetitively, a short length unwound followed by rapid reannealing in successions. We showthat monomeric BLM can "measure" how many base pairs it has unwound, and after a critical length it reverses the unwinding through strand switching and translocating on the opposing strand. Repetitive unwinding persisted even in the presence of hRPA, andinteraction between wild-type BLM and hRPA was necessary for unwinding reinitiation on hRPA-coated DNA. These activities may facilitate BLM processing of stalled replication forks and illegitimately formed recombination intermediates.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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