DNA damage-induced regulatory interplay between DAXX, p53, ATM kinase and Wip1 phosphatase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F15%3A00442490" target="_blank" >RIV/68378050:_____/15:00442490 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.4161/15384101.2014.988019" target="_blank" >http://dx.doi.org/10.4161/15384101.2014.988019</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4161/15384101.2014.988019" target="_blank" >10.4161/15384101.2014.988019</a>

Result language

angličtina

Original language name

DNA damage-induced regulatory interplay between DAXX, p53, ATM kinase and Wip1 phosphatase

Original language description

Death domain-associated protein 6 (DAXX) is a histone chaperone, putative regulator of apoptosis and transcription, and candidate modulator of p53-mediated gene expression following DNA damage. DAXX becomes phosphorylated upon DNA damage, however regulation of this modification, and its relationship to p53 remain unclear. Here we show that in human cells exposed to ionizing radiation or genotoxic drugs etoposide and neocarzinostatin, DAXX became rapidly phosphorylated in an ATM kinase-dependent manner.Our deletion and site-directed mutagenesis experiments identified Serine 564 (S564) as the dominant ATM-targeted site of DAXX, and immunofluorescence experiments revealed localization of S564-phosphorylated DAXX to PML nuclear bodies. Furthermore, usinga panel of human cell types, we identified the p53-regulated Wip1 protein phosphatase as a key negative regulator of DAXX phosphorylation at S564, both in vitro and in cells. Consistent with the emerging oncogenic role of Wip1, its DAXX-d

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GPP305%2F11%2FP683" target="_blank" >GPP305/11/P683: Post-translational modifications of Daxx and their functional relevance in DNA damage response and cellular senescence</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cell Cycle

ISSN

1538-4101

e-ISSN

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Volume of the periodical

14

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

375-387

UT code for WoS article

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EID of the result in the Scopus database

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