Spatial separation of Plk1 phosphorylation and activity

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F15%3A00455583" target="_blank" >RIV/68378050:_____/15:00455583 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.3389/fonc.2015.00132" target="_blank" >http://dx.doi.org/10.3389/fonc.2015.00132</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fonc.2015.00132" target="_blank" >10.3389/fonc.2015.00132</a>

Result language

angličtina

Original language name

Spatial separation of Plk1 phosphorylation and activity

Original language description

Polo-like kinase 1 (P1k1) is one of the major kinases controlling mitosis and cell division. Plk1 is first recruited to the centrosome in S phase, then appears on the kinetochores in late G2, and at the end of mitosis, it translocates to the central spindle. Activation of Plk1 requires phosphorylation of 1210 by Aurora A, an event that critically depends on the co-factor Bora. However, conflicting reports exist as to where Plk1 is first activated. Phosphorylation of 1210 is first observed at the centrosomes, but kinase activity seems to be restricted to the nucleus in the earlier phases of G2. Here, we demonstrate that Plk1 activity manifests itself first in the nucleus using a nuclear FRET-based biosensor for Plk1 activity. However, we find that Borais restricted to the cytoplasm and that Plk1 is phosphorylated on 1210 at the centrosomes. Our data demonstrate that while Plk1 activation occurs on centrosomes, downstream target phosphorylation by Plk1 first occurs in the nucleus. We di

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA13-18392S" target="_blank" >GA13-18392S: Dynamics of DNA damage response in cells</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Oncology

ISSN

2234-943X

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

132

Country of publishing house

CH - SWITZERLAND

Number of pages

8

Pages from-to

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UT code for WoS article

000359144700001

EID of the result in the Scopus database

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