Overexpression and Nucleolar Localization of ?-Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F15%3A00455787" target="_blank" >RIV/68378050:_____/15:00455787 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1097/NEN.0000000000000212" target="_blank" >http://dx.doi.org/10.1097/NEN.0000000000000212</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1097/NEN.0000000000000212" target="_blank" >10.1097/NEN.0000000000000212</a>
Alternative languages
Result language
angličtina
Original language name
Overexpression and Nucleolar Localization of ?-Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma
Original language description
The expression, cellular distribution, and subcellular sorting of the microtubule (MT)-nucleating -tubulin small complex (TuSC) proteins, GCP2 and GCP3, were studied in human glioblastoma cell lines and in clinical tissue samples representing all histologic grades of adult diffuse astrocytic gliomas (n = 54). Quantitative real-time polymerase chain reaction revealed a significant increase in the expression of GCP2 and GCP3 transcripts in glioblastoma cells versus normal human astrocytes; these were associated with higher amounts of both TuSC proteins. GCP2 and GCP3 were concentrated in the centrosomes in interphase glioblastoma cells, but punctate and diffuse localizations were also detected in the cytosol and nuclei/nucleoli. Nucleolar localization was fixation dependent. GCP2 and GCP3 formed complexes with -tubulin in the nucleoli as confirmed by reciprocal immunoprecipitation experiments and immunoelectron microscopy. GCP2 and GCP3 depletion caused accumulation of cells in G(2)/M an
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Neuropathology and Experimental Neurology
ISSN
0022-3069
e-ISSN
—
Volume of the periodical
74
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
723-742
UT code for WoS article
000356937000007
EID of the result in the Scopus database
—