Colocalization coefficients evaluating the distribution of molecular targets in microscopy methods based on pointed patterns

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F16%3A00472647" target="_blank" >RIV/68378050:_____/16:00472647 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00418-016-1467-y" target="_blank" >http://dx.doi.org/10.1007/s00418-016-1467-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00418-016-1467-y" target="_blank" >10.1007/s00418-016-1467-y</a>

Result language

angličtina

Original language name

Colocalization coefficients evaluating the distribution of molecular targets in microscopy methods based on pointed patterns

Original language description

In biomedical studies, the colocalization is commonly understood as the overlap between distinctive labelings in images. This term is usually associated especially with quantitative evaluation of the immunostaining in fluorescence microscopy. On the other hand, the evaluation of the immunolabeling colocalization in the electron microscopy images is still under-investigated and biased by the subjective and non-quantitative interpretation of the image data. We introduce a novel computational technique for quantifying the level of colocalization in pointed patterns. Our approach follows the idea included in the widely used Manders' colocalization coefficients in fluorescence microscopy and represents its counterpart for electron microscopy. In presented methodology, colocalization is understood as the product of the spatial interactions at the single-particle (single-molecule) level. Our approach extends the current significance testing in the immunoelectron microscopy images and establishes the descriptive colocalization coefficients. To demonstrate the performance of the proposed coefficients, we investigated the level of spatial interactions of phosphatidylinositol 4,5-bisphosphate with fibrillarin in nucleoli. We compared the electron microscopy colocalization coefficients with Manders' colocalization coefficients for confocal microscopy and super-resolution structured illumination microscopy. The similar tendency of the values obtained using different colocalization approaches suggests the biological validity of the scientific conclusions. The presented methodology represents a good basis for further development of the quantitative analysis of immunoelectron microscopy data and can be used for studying molecular interactions at the ultrastructural level. Moreover, this methodology can be applied also to the other super-resolution microscopy techniques focused on characterization of discrete pointed structures.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Histochemistry and Cell Biology

ISSN

0948-6143

e-ISSN

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Volume of the periodical

146

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

16

Pages from-to

391-406

UT code for WoS article

000385169600004

EID of the result in the Scopus database

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