Surpassing the Diffraction Limit in Label-Free Optical Microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F24%3A00599726" target="_blank" >RIV/67985882:_____/24:00599726 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22340/24:43929797
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acsphotonics.4c00745" target="_blank" >https://pubs.acs.org/doi/10.1021/acsphotonics.4c00745</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acsphotonics.4c00745" target="_blank" >10.1021/acsphotonics.4c00745</a>
Alternative languages
Result language
angličtina
Original language name
Surpassing the Diffraction Limit in Label-Free Optical Microscopy
Original language description
This perspective article outlines our vision for future directions in label-free super-resolution microscopy. Drawing insights from the success of fluorescence-based super-resolution techniques, we identify parallels in their label-free counterparts and highlight key challenges in the field. We argue here that the progress in optical instrumentation and advanced microscopy methods achieving single-molecule sensitivity, subdiffractional resolution, and high temporal resolution without labels enables observing processes that are either too complex, too fast, or simply not compatible with the labeling. Leveraging a comprehensive review as a foundation, we extrapolate potential developments and, inspired by our recent work, introduce the concept of iSCAT-PAINT combining interferometric scattering microscopy with the concept of PAINT microscopy. This approach resolves fluctuations of interacting single molecules to reveal subdiffractional details of biological structures
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Photonics
ISSN
2330-4022
e-ISSN
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Volume of the periodical
11
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
3907-3921
UT code for WoS article
001300236200001
EID of the result in the Scopus database
2-s2.0-85202491241