Ex vivo tools for the clonal analysis of zebrafish hematopoiesis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F16%3A00473122" target="_blank" >RIV/68378050:_____/16:00473122 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1038/nprot.2016.053" target="_blank" >http://dx.doi.org/10.1038/nprot.2016.053</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/nprot.2016.053" target="_blank" >10.1038/nprot.2016.053</a>

Result language

angličtina

Original language name

Ex vivo tools for the clonal analysis of zebrafish hematopoiesis

Original language description

This protocol describes the ex vivo characterization of zebrafish hematopoietic progenitors. We show how to isolate zebrafish hematopoietic cells for cultivation and differentiation in colony assays in semi-solid media. We also describe procedures for the generation of recombinant zebrafish cytokines and for the isolation of carp serum, which are essential components of the medium required to grow zebrafish hematopoietic cells ex vivo. The outcome of these clonal assays can easily be evaluated using standard microscopy techniques after 3-10 d in culture. In addition, we describe how to isolate individual colonies for further imaging and gene expression profiling. In other vertebrate model organisms, ex vivo assays have been crucial for elucidating the relationships among hematopoietic stem cells (HSCs), progenitor cells and their mature progeny. The present protocol should facilitate such studies on cells derived from zebrafish.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature Protocols

ISSN

1754-2189

e-ISSN

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Volume of the periodical

11

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

1007-1020

UT code for WoS article

000375154300003

EID of the result in the Scopus database

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