Amphioxus functional genomics and the origins of vertebrate gene regulation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F18%3A00502270" target="_blank" >RIV/68378050:_____/18:00502270 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1038/s41586-018-0734-6" target="_blank" >http://dx.doi.org/10.1038/s41586-018-0734-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41586-018-0734-6" target="_blank" >10.1038/s41586-018-0734-6</a>

Result language

angličtina

Original language name

Amphioxus functional genomics and the origins of vertebrate gene regulation

Original language description

Vertebrates have greatly elaborated the basic chordate body plan and evolved highly distinctive genomes that have been sculpted by two whole-genome duplications. Here we sequence the genome of the Mediterranean amphioxus (Branchiostoma lanceolatum) and characterize DNA methylation, chromatin accessibility, histone modifications and transcriptomes across multiple developmental stages and adult tissues to investigate the evolution of the regulation of the chordate genome. Comparisons with vertebrates identify an intermediate stage in the evolution of differentially methylated enhancers, and a high conservation of gene expression and its cis-regulatory logic between amphioxus and vertebrates that occurs maximally at an earlier mid-embryonic phylotypic period. We analyse regulatory evolution after whole-genome duplications, and find that-in vertebrates-over 80% of broadly expressed gene families with multiple paralogues derived from whole-genome duplications have members that restricted their ancestral expression, and underwent specialization rather than subfunctionalization. Counter-intuitively, paralogues that restricted their expression increased the complexity of their regulatory landscapes. These data pave the way for a better understanding of the regulatory principles that underlie key vertebrate innovations.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature

ISSN

0028-0836

e-ISSN

—

Volume of the periodical

564

Issue of the periodical within the volume

7734

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

64-70

UT code for WoS article

000452269400041

EID of the result in the Scopus database

—