Identification of Nodal-dependent enhancer of amphioxus Chordin sufficient to drive gene expression into the chordate dorsal organizer
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F22%3A00564837" target="_blank" >RIV/68378050:_____/22:00564837 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/s00427-022-00698-z" target="_blank" >https://doi.org/10.1007/s00427-022-00698-z</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00427-022-00698-z" target="_blank" >10.1007/s00427-022-00698-z</a>
Alternative languages
Result language
angličtina
Original language name
Identification of Nodal-dependent enhancer of amphioxus Chordin sufficient to drive gene expression into the chordate dorsal organizer
Original language description
The core molecular mechanisms of dorsal organizer formation during gastrulation are highly conserved within the chordate lineage. One of the key characteristics is that Nodal signaling is required for the organizer-specific gene expression. This feature appears to be ancestral, as evidenced by the presence in the most basally divergent chordate amphioxus. To provide a better understanding of the evolution of organizer-specific gene regulation in chordates, we analyzed the cis-regulatory sequence of amphioxus Chordin in the context of the vertebrate embryo. First, we generated stable zebrafish transgenic lines, and by using light-sheet fluorescent microscopy, characterized in detail the expression pattern of GFP driven by the cis-regulatory sequences of amphioxus Chordin. Next, we performed a 5'deletion analysis and identified an enhancer sufficient to drive the expression of the reporter gene into a chordate dorsal organizer. Finally, we found that the identified enhancer element strongly depends on Nodal signaling, which is consistent with the well-established role of this pathway in the regulation of the expression of dorsal organizer-specific genes across chordates. The enhancer identified in our study may represent a suitable simple system to study the interplay of the evolutionarily conserved regulatory mechanisms operating during early chordate development.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA20-25377S" target="_blank" >GA20-25377S: Genetic, transcriptomic and molecular characterization of photoreceptors in the basal chordate amphioxus</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Development Genes and Evolution
ISSN
0949-944X
e-ISSN
1432-041X
Volume of the periodical
232
Issue of the periodical within the volume
5-6
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
137-145
UT code for WoS article
000882788300001
EID of the result in the Scopus database
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