Wnt/beta-catenin signaling is an evolutionarily conserved determinant of chordate dorsal organizer

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F20%3A00538170" target="_blank" >RIV/68378050:_____/20:00538170 - isvavai.cz</a>
Result on the web
<a href="https://elifesciences.org/articles/56817" target="_blank" >https://elifesciences.org/articles/56817</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.7554/eLife.56817" target="_blank" >10.7554/eLife.56817</a>

Result language
angličtina
Original language name
Wnt/beta-catenin signaling is an evolutionarily conserved determinant of chordate dorsal organizer
Original language description
Deciphering the mechanisms of axis formation in amphioxus is a key step to understanding the evolution of chordate body plan. The current view is that Nodal signaling is the only factor promoting the dorsal axis specification in the amphioxus, whereas Wnt/beta-catenin signaling plays no role in this process. Here, we re-examined the role of Wnt/beta catenin signaling in the dorsal/ventral patterning of amphioxus embryo. We demonstrated that the spatial activity of Wnt/beta-catenin signaling is located in presumptive dorsal cells from cleavage to gastrula stage, and provided functional evidence that Wnt/beta-catenin signaling is necessary for the specification of dorsal cell fate in a stage-dependent manner. Microinjection of Wnt8 and Wnt11 mRNA induced ectopic dorsal axis in neurulae and larvae. Finally, we demonstrated that Nodal and Wnt/beta-catenin signaling cooperate to promote the dorsal-specific gene expression in amphioxus gastrula. Our study reveals high evolutionary conservation of dorsal organizer formation in the chordate lineage.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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