Distinct phenotypes and ´bystander' effects of senescent tumour cells induced by docetaxel or immunomodulatory cytokines

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F18%3A00502293" target="_blank" >RIV/68378050:_____/18:00502293 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.3892/ijo.2018.4553" target="_blank" >http://dx.doi.org/10.3892/ijo.2018.4553</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/ijo.2018.4553" target="_blank" >10.3892/ijo.2018.4553</a>

Result language

angličtina

Original language name

Distinct phenotypes and ´bystander' effects of senescent tumour cells induced by docetaxel or immunomodulatory cytokines

Original language description

Cellular senescence is the process of the permanent proliferative arrest of cells in response to various inducers. It is accompanied by typical morphological changes, in addition to the secretion of bioactive molecules, including proinflammatory cytokines and chemokines [known as the senescence-associated secretory phenotype (SASP)]. Thus, senescent cells may affect their local environment and induce a so-called bystander' senescence through the state of SASP. The phenotypes of senescent cells are determined by the type of agent inducing cellular stress and the cell lineages. To characterise the phenotypes of senescent cancer cells, two murine cell lines were employed in the present study: TC-1 and B16F10 (B16) cells. Two distinct senescence inductors were used: Chemotherapeutic agent docetaxel (DTX) and a combination of immunomodulatory cytokines, including interferon (IFN) and tumour necrosis factor (TNF). It was demonstrated that DTX induced senescence in TC-1 and B16 tumour cell lines, which was demonstrated by growth arrest, positivegalactosidase staining, increased p21(Waf1) (p21) expression and the typical SASP capable of inducing a bystander' senescence. By contrast, treatment with a combination of T helper cell 1 cytokines, IFN and TNF, induced proliferation arrest only in B16 cells. Despite the presence of certain characteristic features resembling senescent cells (proliferation arrest, morphological changes and increased p21 expression), these cells were able to form tumours in vivo and started to proliferate upon cytokine withdrawal. In addition, B16 cells were not able to induce a bystander' senescence. In summary, the present study described cell line- and treatment- associated differences in the phenotypes of senescent cells that may be relevant in optimization of cancer chemo- and immunotherapy.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Oncology

ISSN

1019-6439

e-ISSN

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Volume of the periodical

53

Issue of the periodical within the volume

5

Country of publishing house

GR - GREECE

Number of pages

13

Pages from-to

1997-2009

UT code for WoS article

000447703300014

EID of the result in the Scopus database

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