Luciferase-based assays for monitoring G-protein coupled receptors signaling

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F19%3A00502895" target="_blank" >RIV/68378050:_____/19:00502895 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Luciferase-based assays for monitoring G-protein coupled receptors signaling
Original language description
Mutations in G-protein coupled receptors (GPCRs) affecting their function and signaling capabilities were shown to be associated with many human diseases. GPCRs are thus one of the most promising therapeutic targets. Establishment of assays enabling high throughput screenings of GPCR signaling are therefore highly warranted. In this review we provide overview of new strategies used for examination of GPCR signaling cascades. We focus mainly on recent studies using modified cyclic AMP sensitive luciferase enzyme. These assays enable detection of GPCR activation in living cells in real time. Moreover, this experimental design allows simultaneous and repeated measuring of GPCR association with any of possible downstream signaling cascades in one experimental setup. We summarize also papers presenting utilization of luciferase-based assays for analysis of opsins, light sensitive GPCRs, whose activation occurs in range of milliseconds.
Czech name
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Czech description
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Project
<a href="/en/project/LO1220" target="_blank" >LO1220: CZ-OPENSCREEN: National infrastructure for chemical biology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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