LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00557640" target="_blank" >RIV/67985904:_____/22:00557640 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/22:00077656 RIV/00216224:14110/22:00125756
Result on the web
<a href="https://www.life-science-alliance.org/content/5/8/e202201421" target="_blank" >https://www.life-science-alliance.org/content/5/8/e202201421</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.26508/lsa.202201421" target="_blank" >10.26508/lsa.202201421</a>
Alternative languages
Result language
angličtina
Original language name
LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters
Original language description
Luciferase reporter assays represent a simple and sensitive experimental system in cell and molecular biology to study multiple biological processes. However, the application of these assays is often limited by the costs of conventional luminometer instruments and the versatility of their use in different experimental conditions. Therefore, we aimed to develop a small, affordable luminometer allowing continuous measurement of luciferase activity, designed for inclusion into various kinds of tissue culture incubators. Here, we introduce LuminoCell-an open-source platform for the construction of an affordable, sensitive, and portable luminometer capable of real-time monitoring in-cell luciferase activity. The LuminoCell costs $40, requires less than 1 h to assemble, and it is capable of performing real-time sensitive detection of both magnitude and duration of the activity of major signalling pathways in cell cultures, including receptor tyrosine kinases (EGF and FGF), WNT/beta-catenin, and NF-kappa B. In addition, we show that the LuminoCell is suitable to be used in cytotoxicity assays as well as for monitoring periodic circadian gene expression.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Life Science Alliance
ISSN
2575-1077
e-ISSN
2575-1077
Volume of the periodical
5
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
e202201421
UT code for WoS article
000793098700002
EID of the result in the Scopus database
2-s2.0-85128797977