Development of a novel gene mutagenesis and protein tagging strategy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F19%3A00523226" target="_blank" >RIV/68378050:_____/19:00523226 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Development of a novel gene mutagenesis and protein tagging strategy
Original language description
Random gene trap mutagenesis is a powerful technique to rapidly generate a library of mutated genes while also providing a reporter for their respective expression. One problem is that efficient mutagenesis requires insertion of the vector at the beginning of a gene, while an informative subcellular reporter requires insertion at the end of a gene. Here we introduce a novel gene trap approach that overcomes this issue, creating a multifunctional allele that permits both effective mutagenesis and endogenous protein tagging.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Advances in Disease Models
ISBN
978-80-88011-06-4
Number of pages of the result
9
Pages from-to
106-114
Number of pages of the book
310
Publisher name
OPTIO CZ
Place of publication
Praha
UT code for WoS chapter
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