SUPER-RESOLUTION LIGHT MICROSCOPY: STED microscope fine laser co-alignment influences the final resolution and SNR

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F19%3A00523243" target="_blank" >RIV/68378050:_____/19:00523243 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
SUPER-RESOLUTION LIGHT MICROSCOPY: STED microscope fine laser co-alignment influences the final resolution and SNR
Original language description
The super-resolution (SR) methods are rapidly developing approaches in light microscopy field. The Stimulated emission depletion (STED) microscopy is SR method based on confocal scanning microscope. During the acquisition scanning, two lasers – excitation and depletion – are co-aligned into one single spot in the sample. For the maximal efficiency of the SR method, the proper co-alignment of used laser beams is crucial. The article explains the STED microscope operator recommended routine for the alignment. Simple experiments demonstrate significantly decreased number of incoming photons in the misaligned system. It naturally leads to the lower resolution during the acquisition and these important aspects are discussed within the article.
Czech name
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Czech description
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Project
<a href="/en/project/LO1419" target="_blank" >LO1419: Biomodels for Health - Center for model organisms</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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