Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F18%3A00506542" target="_blank" >RIV/61388955:_____/18:00506542 - isvavai.cz</a>

Result on the web

<a href="http://hdl.handle.net/11104/0297773" target="_blank" >http://hdl.handle.net/11104/0297773</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41467-018-05963-2" target="_blank" >10.1038/s41467-018-05963-2</a>

Result language

angličtina

Original language name

Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures

Original language description

Imaging of nuclear structures within intact eukaryotic nuclei is imperative to understand the effect of chromatin folding on genome function. Recent developments of super-resolution fluorescence microscopy techniques combine high specificity, sensitivity, and less-invasive sample preparation procedures with the sub-diffraction spatial resolution required to image chromatin at the nanoscale. Here, we present a method to enhance the spatial resolution of a stimulated-emission depletion (STED) microscope based only on the modulation of the STED intensity during the acquisition of a STED image. This modulation induces spatially encoded variations of the fluorescence emission that can be visualized in the phasor plot and used to improve and quantify the effective spatial resolution of the STED image. We show that the method can be used to remove direct excitation by the STED beam and perform dual color imaging. We apply this method to the visualization of transcription and replication foci within intact nuclei of eukaryotic cells.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10403 - Physical chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature Communications

ISSN

2041-1723

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

AUG 2018

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

3415

UT code for WoS article

000442594800017

EID of the result in the Scopus database

2-s2.0-85057564860