The African Swine Fever Virus Transcriptome
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F20%3A00524183" target="_blank" >RIV/68378050:_____/20:00524183 - isvavai.cz</a>
Result on the web
<a href="https://jvi.asm.org/content/94/9/e00119-20" target="_blank" >https://jvi.asm.org/content/94/9/e00119-20</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/JVI.00119-20" target="_blank" >10.1128/JVI.00119-20</a>
Alternative languages
Result language
angličtina
Original language name
The African Swine Fever Virus Transcriptome
Original language description
African swine fever virus (ASFV) causes hemorrhagic fever in domestic pigs, presenting the biggest global threat to animal farming in recorded history. Despite the importance of ASFV, little is known about the mechanisms and regulation of ASFV transcription. Using RNA sequencing methods, we have determined total RNA abundance, transcription start sites, and transcription termination sites at single-nucleotide resolution. This allowed us to characterize DNA consensus motifs of early and late ASFV core promoters, as well as a polythymidylate sequence determinant for transcription termination. Our results demonstrate that ASFV utilizes alternative transcription start sites between early and late stages of infection and that ASFV RNA polymerase (RNAP) undergoes promoter-proximal transcript slippage at 5' ends of transcription units, adding quasitemplated AU- and AUAU-5' extensions to mRNAs. Here, we present the first much-needed genome-wide transcriptome study that provides unique insight into ASFV transcription and serves as a resource to aid future functional analyses of ASFV genes which are essential to combat this devastating disease.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Virology
ISSN
0022-538X
e-ISSN
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Volume of the periodical
94
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
22
Pages from-to
e00119-20
UT code for WoS article
000527363300005
EID of the result in the Scopus database
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