Distinct populations of crypt-associated fibroblasts act as signaling hubs to control colon homeostasis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F20%3A00538133" target="_blank" >RIV/68378050:_____/20:00538133 - isvavai.cz</a>
Result on the web
<a href="https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3001032" target="_blank" >https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3001032</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pbio.3001032" target="_blank" >10.1371/journal.pbio.3001032</a>
Alternative languages
Result language
angličtina
Original language name
Distinct populations of crypt-associated fibroblasts act as signaling hubs to control colon homeostasis
Original language description
Despite recent progress in recognizing the importance of mesenchymal cells for the homeostasis of the intestinal system, the current picture of how these cells communicate with the associated epithelial layer remains unclear. To describe the relevant cell populations in an unbiased manner, we carried out a single-cell transcriptome analysis of the adult murine colon, producing a high-quality atlas of matched colonic epithelium and mesenchyme. We identify two crypt-associated colonic fibroblast populations that are demarcated by different strengths of platelet-derived growth factor receptor A (Pdgfra) expression. Crypt-bottom fibroblasts (CBFs), close to the intestinal stem cells, express low levels of Pdgfra and secrete canonical Wnt ligands, Wnt potentiators, and bone morphogenetic protein (Bmp) inhibitors. Crypt-top fibroblasts (CTFs) exhibit high Pdgfra levels and secrete noncanonical Wnts and Bmp ligands. While the Pdgfra(low) cells maintain intestinal stem cell proliferation, the Pdgfra(high) cells induce differentiation of the epithelial cells. Our findings enhance our understanding of the crosstalk between various colonic epithelial cells and their associated mesenchymal signaling hubs along the crypt axis-placing differential Pdgfra expression levels in the spotlight of intestinal fibroblast identity.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA18-21466S" target="_blank" >GA18-21466S: Wnt ligands and Wnt-secreting cells for intestinal homeostasis, regeneration and colon cancer: a complex world beyond the cancer-activating mutation</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
P L o S Biology
ISSN
1544-9173
e-ISSN
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Volume of the periodical
18
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
e3001032
UT code for WoS article
000600098800002
EID of the result in the Scopus database
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