Identification of retinal ganglion cell types and brain nuclei expressing the transcription factor Brn3c/Pou4f3 using a Cre recombinase knock-in allele
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F21%3A00539692" target="_blank" >RIV/68378050:_____/21:00539692 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1002/cne.25065" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/cne.25065</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cne.25065" target="_blank" >10.1002/cne.25065</a>
Alternative languages
Result language
angličtina
Original language name
Identification of retinal ganglion cell types and brain nuclei expressing the transcription factor Brn3c/Pou4f3 using a Cre recombinase knock-in allele
Original language description
Members of the POU4F/Brn3 transcription factor family have an established role in the development of retinal ganglion cell (RGCs) types, the main transducers of visual information from the mammalian eye to the brain. Our previous work using sparse random recombination of a conditional knock-in reporter allele expressing alkaline phosphatase (AP) and intersectional genetics had identified three types of Brn3c positive (Brn3c(+)) RGCs. Here, we describe a novel Brn3c(Cre) mouse allele generated by serial Dre to Cre recombination and use it to explore the expression overlap of Brn3c with Brn3a and Brn3b and the dendritic arbor morphologies and visual stimulus response properties of Brn3c(+) RGC types. Furthermore, we explore brain nuclei that express Brn3c or receive input from Brn3c(+) neurons. Our analysis reveals a much larger number of Brn3c(+) RGCs and more diverse set of RGC types than previously reported. Most RGCs expressing Brn3c during development are still Brn3c positive in the adult, and all express Brn3a while only about half express Brn3b. Genetic Brn3c-Brn3b intersection reveals an area of increased RGC density, extending from dorsotemporal to ventrolateral across the retina and overlapping with the mouse binocular field of view. In addition, we report a Brn3c(+) RGC projection to the thalamic reticular nucleus, a visual nucleus that was not previously shown to receive retinal input. Furthermore, Brn3c(+) neurons highlight a previously unknown subdivision of the deep mesencephalic nucleus. Thus, our newly generated allele provides novel biological insights into RGC type classification, brain connectivity, and cytoarchitectonic.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30103 - Neurosciences (including psychophysiology)
Result continuities
Project
<a href="/en/project/GA18-20759S" target="_blank" >GA18-20759S: The role of Meis homeobox genes in retina development</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Comparative Neurology
ISSN
0021-9967
e-ISSN
1096-9861
Volume of the periodical
529
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
28
Pages from-to
25065
UT code for WoS article
000587888900001
EID of the result in the Scopus database
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