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Efficient allele conversion in mouse zygotes and primary cells based on electroporation of Cre protein

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F20%3A00540328" target="_blank" >RIV/68378050:_____/20:00540328 - isvavai.cz</a>

  • Alternative codes found

    RIV/86652036:_____/20:00540328

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/abs/pii/S1046202320301146?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S1046202320301146?via%3Dihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.ymeth.2020.07.005" target="_blank" >10.1016/j.ymeth.2020.07.005</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Efficient allele conversion in mouse zygotes and primary cells based on electroporation of Cre protein

  • Original language description

    Cre-loxP recombination system is a powerful tool for genome engineering. One of its applications is found in genetic mouse models that often require to induce Cre recombination in preimplantation embryos. Here, we describe a technically simple, affordable and highly efficient protocol for Cre protein delivery into mouse zygotes by electroporation. We show that electroporation based delivery of Cre has no negative impact on embryo survival and the method can be easily combined with in vitro fertilization resulting in a significantly faster generation of desired models. Lastly, we demonstrate that Cre protein electroporation is suitable for allelic conversion in primary cells derived from conditional mouse models.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Methods

  • ISSN

    1046-2023

  • e-ISSN

    1095-9130

  • Volume of the periodical

    S1046-2023

  • Issue of the periodical within the volume

    20

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

    30114-6

  • UT code for WoS article

    000660517600010

  • EID of the result in the Scopus database