Efficient allele conversion in mouse zygotes and primary cells based on electroporation of Cre protein
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F21%3A00540328" target="_blank" >RIV/68378050:_____/21:00540328 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/20:00540328
Result on the web
<a href="https://www.sciencedirect.com/science/article/abs/pii/S1046202320301146?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S1046202320301146?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ymeth.2020.07.005" target="_blank" >10.1016/j.ymeth.2020.07.005</a>
Alternative languages
Result language
angličtina
Original language name
Efficient allele conversion in mouse zygotes and primary cells based on electroporation of Cre protein
Original language description
Cre-loxP recombination system is a powerful tool for genome engineering. One of its applications is found in genetic mouse models that often require to induce Cre recombination in preimplantation embryos. Here, we describe a technically simple, affordable and highly efficient protocol for Cre protein delivery into mouse zygotes by electroporation. We show that electroporation based delivery of Cre has no negative impact on embryo survival and the method can be easily combined with in vitro fertilization resulting in a significantly faster generation of desired models. Lastly, we demonstrate that Cre protein electroporation is suitable for allelic conversion in primary cells derived from conditional mouse models.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Methods
ISSN
1046-2023
e-ISSN
1095-9130
Volume of the periodical
191
Issue of the periodical within the volume
JUL
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
87-94
UT code for WoS article
000660517600010
EID of the result in the Scopus database
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