Maternal/zygotic knockout and droplet digital polymerase chain reaction analysis question the role of activin A in preimplantation mouse embryo development
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F25%3A00644596" target="_blank" >RIV/67985904:_____/25:00644596 - isvavai.cz</a>
Result on the web
<a href="https://watermark02.silverchair.com/ioaf189.pdf" target="_blank" >https://watermark02.silverchair.com/ioaf189.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/biolre/ioaf189" target="_blank" >10.1093/biolre/ioaf189</a>
Alternative languages
Result language
angličtina
Original language name
Maternal/zygotic knockout and droplet digital polymerase chain reaction analysis question the role of activin A in preimplantation mouse embryo development
Original language description
For many years, activin A, encoded by Inhba, has been thought to be present in both mouse and human oocytes and preimplantation embryos. However, its deficiency does not impede the proper embryonic development of the embryo until birth. It has been suggested that the lack of a phenotype in zygotic knockout embryos may be masked by the presence of maternal protein deposited in the oocyte during oogenesis or provided from the reproductive tract. Therefore, to explore whether maternally supplied activin A is required for embryo development, we carried out a conditional Inhba knockout in oocytes using Zp3-Cre/LoxP strategy. By examining Inhba maternal and maternal/zygotic knockout embryos, individually recorded using time-lapse imaging, immunostained, and genotyped, we revealed that the maternal pool of activin A affects the dynamics of mouse preimplantation development. These alterations are accompanied by impaired mitochondrial activity in oocytes. Surprisingly, using the droplet digital polymerase chain reaction approach, we provided evidence that the Inhba mRNA of zygotic origin is undetectable in mouse embryos.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biology of Reproduction
ISSN
0006-3363
e-ISSN
1529-7268
Volume of the periodical
113
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
1375-1389
UT code for WoS article
001562362700001
EID of the result in the Scopus database
2-s2.0-105025017556