The walevet-based denoising of images in fiji, with exampleapplications in structured illumination microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F21%3A00542949" target="_blank" >RIV/68378050:_____/21:00542949 - isvavai.cz</a>

Result on the web

<a href="https://www.ias-iss.org/ojs/IAS/article/view/2432" target="_blank" >https://www.ias-iss.org/ojs/IAS/article/view/2432</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5566/ias.2432" target="_blank" >10.5566/ias.2432</a>

Result language

angličtina

Original language name

The walevet-based denoising of images in fiji, with exampleapplications in structured illumination microscopy

Original language description

Filtration of super-resolved microscopic images brings often troubles with removing undesired image parts like, e.g., noise, inhomogenous background and reconstruction artifacts. Standard filtration techniques, e.g., convolution- or Fourier transform-based methods are not always appropriate, since they may lower image resolution that was acquired by hi-tech and expensive microscopy systems. Thus, in this article it is proposed to filter such images using discrete wavelet transform (DWT). Newly developed Wavelet_ Denoise plugin for free available Fiji software package demonstrates important possibilities of applying DWT to images: Decomposition of a filtered picture using various wavelet filters and levels of details with showing decomposed images and visualization of effects of back transformation of the picture with chosen level of suppression or denoising of wavelet coefficients. The Fiji framework allows, for example, using a plethora of various microscopic image formats for data opening, users can easily install the plugin through a menu command and the plugin supports processing 3D images in Z-stacks. The application of the plugin for removal of reconstruction artifacts and undesirable background in images acquired by super-resolved structured illumination microscopy is demonstrated as well.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20206 - Computer hardware and architecture

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Image Analysis and Stereology

ISSN

1580-3139

e-ISSN

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Volume of the periodical

40

Issue of the periodical within the volume

1

Country of publishing house

SK - SLOVAKIA

Number of pages

14

Pages from-to

3-16

UT code for WoS article

000639563600002

EID of the result in the Scopus database

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