All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

Phospholipid identity of the ene expression compartments

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F21%3A00555749" target="_blank" >RIV/68378050:_____/21:00555749 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Phospholipid identity of the ene expression compartments

  • Original language description

    Single-molecule localization microscopy (SMLM) provided an unprecedented insight into the sub-nuclear organization of proteins and nucleic acids. However, the role of the nuclear lipids in the establishment of the functional nuclear architecture, apart from the nuclear envelope, has been neglected. Nevertheless, the roles in gene expression of the nuclear lipids and phosphatidylinositol phosphates (PIPs) in particular started to emerge. Therefore, we implemented and optimized the SMLM-based approach for the quantitative evaluation of the nuclear PIP distribution while preserving the context of nuclear architecture. We have quantitatively characterized the spatial distribution of nuclear phosphatidylinositol 4,5- and 3,4-bisphosphate (PI(4,5)P2 and PI(3,4)P2, resp.) and showed that PI(4,5)P2 and PI(3,4)P2 localize within matrix of the nuclear speckle marker SON and in the nucleoplasmic foci. Moreover, we found PI(4,5)P2 and PI(3,4)P2 in the close proximity with the subset of RNAPII foci either in the nucleoplasm or at nuclear speckles. We started to investigate the cross-talk between nucleoplasmic and nuclear speckle-associated PI(4,5)P2 and PI(3,4)P2 pools and their possible roles in the regulation of RNAPII transcription. Our preliminary data suggest that upon transcription inhibition PI(4,5)P2 and PI(3,4)P2 accumulate within nuclear speckles. Therefore, nuclear speckles could play a role in the buffering of the nuclear PIP levels and thereby possibly regulate RNAPII transcription.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

  • OECD FORD branch

    10601 - Cell biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů