Unraveling the phospholipid identity of the gene expression compartments by single molecule localization microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F21%3A00555844" target="_blank" >RIV/68378050:_____/21:00555844 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/21:00555845

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Unraveling the phospholipid identity of the gene expression compartments by single molecule localization microscopy

Original language description

Current models of gene expression acknowledge protein clustering and formation of transcriptional condensates as a driving force of gene expression. These models are mostly based on single-molecule localization microscopy (SMLM) which provided an unprecedented insight into the sub-nuclear organization of proteins and nucleic acids. However, the roles of nuclear lipids in the establishment of the functional nuclear architecture, apart from the nuclear envelope, has been neglected. Nevertheless, accumulating evidence suggests the involvement of nuclear lipids and particularly of phosphatidylinositol phosphates (PIPs) in gene expression. We used quantitative SMLM for the evaluation of the nuclear PIP distribution while preserving the context of nuclear architecture. We showed phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2) and phosphatidylinositol 4-monophosphate (PI(4)P) within nuclear speckles and in the nucleoplasmic foci. Moreover, we found PI(4,5)P2 and PI(3,4)P2 in the close proximity with the subset of RNA polymerase II (RNAPII) foci either in the nucleoplasm or nuclear speckles. We continue to uncover the PIP fingerprints in the subsequent stages of RNAPII transcription. Our efforts aim at elucidating the roles of nuclear PIPs in the compartmentalization of gene expression.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů