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KNOCK-ING ON HETEROCHROMATIN: STABILITY OF GAMMARETROVIRAL EXPRESSION AFTER INTEGRATION RETARGETING

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F22%3A00567916" target="_blank" >RIV/68378050:_____/22:00567916 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.ccsss.cz/index.php/ccsss/issue/view/37/67" target="_blank" >http://www.ccsss.cz/index.php/ccsss/issue/view/37/67</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    KNOCK-ING ON HETEROCHROMATIN: STABILITY OF GAMMARETROVIRAL EXPRESSION AFTER INTEGRATION RETARGETING

  • Original language description

    Retroviruses integrate their genome into the genome of the infected cell. Integrated proviruses form an inseparable part of the host genome and express viral genes effectively. Epigenetic silencing may however disrupt the proviral expression. We have previously shown that retroviruses differ in sensitivity to epigenetic silencing and that the features at the integration site can affect the stability of proviral expression. Vectors derived from the murine leukemia virus (MLV) –widely used representative of the gammaretrovirus genus –performed as the most efficient retrovirus-derived vector in human cells. Here, we further investigate the gammaretroviral expression in human cells.We constructed retroviral vectors from diverse gammaretroviruses and transduced the human K562 cell line. We further tested if disruption of natural promoter/enhancer targeting affects the gammaretroviral expression. Our results indicate that the vectors express transduced genes stably in time irrespective of integration preference. We further corroborated the gammaretroviral expression stability in heterochromatin by CRISPR-directed knock-in into predicted lamin-associated domains.Our results demonstrate the general ability of gammaretroviruses to stably express transduced genes in human cells. Moreover, we show that other-than-MLV gammaretroviruses may be used for the construction of heterochromatin-resistant expression vectors for transgenesis.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

  • OECD FORD branch

    10607 - Virology

Result continuities

  • Project

    <a href="/en/project/LX22NPO5103" target="_blank" >LX22NPO5103: National Institute of Virology and Bacteriology</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů