Hematopoietic stem cells undergo a lymphoid to myeloid switch in early stages of emergency granulopoiesis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F23%3A00579429" target="_blank" >RIV/68378050:_____/23:00579429 - isvavai.cz</a>

Alternative codes found

RIV/86652036:_____/23:00579429 RIV/00216208:11130/23:10470164 RIV/00216208:11310/23:10470164

Result on the web

<a href="https://www.embopress.org/doi/epdf/10.15252/embj.2023113527?src=getftr" target="_blank" >https://www.embopress.org/doi/epdf/10.15252/embj.2023113527?src=getftr</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15252/embj.2023113527" target="_blank" >10.15252/embj.2023113527</a>

Result language

angličtina

Original language name

Hematopoietic stem cells undergo a lymphoid to myeloid switch in early stages of emergency granulopoiesis

Original language description

Emergency granulopoiesis is the enhanced and accelerated production of granulocytes that occurs during acute infection. The contribution of hematopoietic stem cells (HSCs) to this process was reported, however, how HSCs participate in emergency granulopoiesis remains elusive. Here, using a mouse model of emergency granulopoiesis we observe transcriptional changes in HSCs as early as 4 h after lipopolysaccharide (LPS) administration. We observe that the HSC identity is changed towards a myeloid-biased HSC and show that CD201 is enriched in lymphoid-biased HSCs. While CD201 expression under steady-state conditions reveals a lymphoid bias, under emergency granulopoiesis loss of CD201 marks the lymphoid-to-myeloid transcriptional switch. Mechanistically, we determine that lymphoid-biased CD201+ HSCs act as a first response during emergency granulopoiesis due to direct sensing of LPS by TLR4 and downstream activation of NF-kappa Beta signaling. The myeloid-biased CD201- HSC population responds indirectly during an acute infection by sensing G-CSF, increasing STAT3 phosphorylation, and upregulating LAP/LAP* C/EBP beta isoforms. In conclusion, HSC subpopulations support early phases of emergency granulopoiesis due to their transcriptional rewiring from a lymphoid-biased to myeloid-biased population and thus establishing alternative paths to supply elevated numbers of granulocytes.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

EMBO Journal

ISSN

0261-4189

e-ISSN

1460-2075

Volume of the periodical

42

Issue of the periodical within the volume

23

Country of publishing house

US - UNITED STATES

Number of pages

18

Pages from-to

e113527

UT code for WoS article

001083429900001

EID of the result in the Scopus database

2-s2.0-85174320525