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Behavioral Characterization of an Angelman Syndrome Mouse Model

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F23%3A00581626" target="_blank" >RIV/68378050:_____/23:00581626 - isvavai.cz</a>

  • Result on the web

    <a href="https://www.jove.com/t/65182/behavioral-characterization-of-an-angelman-syndrome-mouse-model" target="_blank" >https://www.jove.com/t/65182/behavioral-characterization-of-an-angelman-syndrome-mouse-model</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3791/65182" target="_blank" >10.3791/65182</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Behavioral Characterization of an Angelman Syndrome Mouse Model

  • Original language description

    This manuscript describes a battery of behavioral tests available to characterize Angelman syndrome (AS)-like phenotypes in an established murine model of AS. We use the rotarod learning paradigm, detailed gait analysis, and nest building test to detect and characterize animal motor impairments. We test animal emotionality in the open field and elevated plus maze tests, as well as the affect in the tail suspension test. When AS mice are tested in the open field test, the results should be interpreted with care, since motor dysfunctions influence mouse behavior in the maze and alter activity scores. The reproducibility and effectiveness of the presented behavioral tests has already been validated in several independent Uba3a mouse lines with different knockout variants, establishing this set of tests as an excellent validation tool in AS research. Models with the relevant construct and face validity will warrant further investigations to elucidate the pathophysiology of the disease and grant the development of causal treatments.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Jove-Journal of Visualized Experiments

  • ISSN

    1940-087X

  • e-ISSN

  • Volume of the periodical

    2023

  • Issue of the periodical within the volume

    200

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    18

  • Pages from-to

    e65182

  • UT code for WoS article

  • EID of the result in the Scopus database

    2-s2.0-85175006976