A closed 3D printed microfluidic device for automated growth and differentiation of cerebral organoids from single-cell suspension
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F24%3A00597827" target="_blank" >RIV/68378050:_____/24:00597827 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/24:00137084 RIV/00159816:_____/24:00081680
Result on the web
<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/biot.202400240" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/biot.202400240</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/biot.202400240" target="_blank" >10.1002/biot.202400240</a>
Alternative languages
Result language
angličtina
Original language name
A closed 3D printed microfluidic device for automated growth and differentiation of cerebral organoids from single-cell suspension
Original language description
The development of 3D organoids has provided a valuable tool for studying human tissue and organ development in vitro. Cerebral organoids, in particular, offer a unique platform for investigating neural diseases. However, current methods for generating cerebral organoids suffer from limitations such as labor-intensive protocols and high heterogeneity among organoids. To address these challenges, we present a microfluidic device designed to automate and streamline the formation and differentiation of cerebral organoids. The device utilizes microwells with two different shapes to promote the formation of a single aggregate per well and incorporates continuous medium flow for optimal nutrient exchange. In silico simulations supported the effectiveness of the microfluidic chip in replicating cellular microenvironments. Our results demonstrate that the microfluidic chip enables uniform growth of cerebral organoids, significantly reducing the hands-on time required for maintenance. Importantly, the performance of the microfluidic system is comparable to the standard 96-well plate format even when using half the amount of culture medium, and the resulting organoids exhibit substantially developed neuroepithelial buds and cortical structures. This study highlights the potential of custom-designed microfluidic technology in improving the efficiency of cerebral organoid culture.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biotechnology Journal
ISSN
1860-6768
e-ISSN
1860-7314
Volume of the periodical
19
Issue of the periodical within the volume
8
Country of publishing house
DE - GERMANY
Number of pages
3
Pages from-to
e2400240
UT code for WoS article
001303094800001
EID of the result in the Scopus database
2-s2.0-85202649553