Tumor Necrosis Factor-Alpha Modulates Expression of Genes Involved in Cytokines and Chemokine Pathways in Proliferative Myoblast Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F24%3A00604438" target="_blank" >RIV/68378050:_____/24:00604438 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2073-4409/13/13/1161" target="_blank" >https://www.mdpi.com/2073-4409/13/13/1161</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/cells13131161" target="_blank" >10.3390/cells13131161</a>
Alternative languages
Result language
angličtina
Original language name
Tumor Necrosis Factor-Alpha Modulates Expression of Genes Involved in Cytokines and Chemokine Pathways in Proliferative Myoblast Cells
Original language description
Skeletal muscle regeneration after injury is a complex process involving inflammatory signaling and myoblast activation. Pro-inflammatory cytokines like tumor necrosis factor-alpha (TNF-alpha) are key mediators, but their effects on gene expression in proliferating myoblasts are unclear. We performed the RNA sequencing of TNF-alpha treated C2C12 myoblasts to elucidate the signaling pathways and gene networks regulated by TNF-alpha during myoblast proliferation. The TNF-alpha (10 ng/mL) treatment of C2C12 cells led to 958 differentially expressed genes compared to the controls. Pathway analysis revealed significant regulation of TNF-alpha signaling, along with the chemokine and IL-17 pathways. Key upregulated genes included cytokines (e.g., IL-6), chemokines (e.g., CCL7), and matrix metalloproteinases (MMPs). TNF-alpha increased myogenic factor 5 (Myf5) but decreased MyoD protein levels and stimulated the release of MMP-9, MMP-10, and MMP-13. TNF-alpha also upregulates versican and myostatin mRNA. Overall, our study demonstrates the TNF-alpha modulation of distinct gene expression patterns and signaling pathways that likely contribute to enhanced myoblast proliferation while suppressing premature differentiation after muscle injury. Elucidating the mechanisms involved in skeletal muscle regeneration can aid in the development of regeneration-enhancing therapeutics.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30204 - Oncology
Result continuities
Project
<a href="/en/project/LM2023036" target="_blank" >LM2023036: Czech Centre for Phenogenomics</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cells
ISSN
2073-4409
e-ISSN
2073-4409
Volume of the periodical
13
Issue of the periodical within the volume
13
Country of publishing house
CH - SWITZERLAND
Number of pages
19
Pages from-to
1161
UT code for WoS article
001266500200001
EID of the result in the Scopus database
2-s2.0-85198384707