Clusterin Deficiency Promotes Cellular Senescence in Human Astrocytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F25%3A00602526" target="_blank" >RIV/68378050:_____/25:00602526 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/25:10499226
Result on the web
<a href="https://link.springer.com/article/10.1007/s12035-024-04650-2" target="_blank" >https://link.springer.com/article/10.1007/s12035-024-04650-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12035-024-04650-2" target="_blank" >10.1007/s12035-024-04650-2</a>
Alternative languages
Result language
angličtina
Original language name
Clusterin Deficiency Promotes Cellular Senescence in Human Astrocytes
Original language description
The glycoprotein clusterin (CLU) is involved in cell proliferation and DNA damage repair and is highly expressed in tumor cells. Here, we aimed to investigate the effects of CLU dysregulation on two human astrocytic cell lines: CCF-STTG1 astrocytoma cells and SV-40 immortalized normal human astrocytes. We observed that suppression of CLU expression by RNA interference inhibited cell proliferation, triggered the DNA damage response, and resulted in cellular senescence in both cell types tested. To further investigate the underlying mechanism behind these changes, we measured reactive oxygen species, assessed mitochondrial function, and determined selected markers of the senescence-associated secretory phenotype. Our results suggest that CLU deficiency triggers oxidative stress-mediated cellular senescence associated with pronounced alterations in mitochondrial membrane potential, mitochondrial mass, and expression levels of OXPHOS complex I, II, III and IV, indicating mitochondrial dysfunction. This report shows the important role of CLU in cell cycle maintenance in astrocytes. Based on these data, targeting CLU may serve as a potential therapeutic approach valuable for treating gliomas.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Neurobiology
ISSN
0893-7648
e-ISSN
1559-1182
Volume of the periodical
62
Issue of the periodical within the volume
5
Country of publishing house
DE - GERMANY
Number of pages
13
Pages from-to
5774-5786
UT code for WoS article
001368823900001
EID of the result in the Scopus database
2-s2.0-85211450466