Live-cell magnetic manipulation of recycling endosomes reveals their direct effect on actin protrusions to promote invasive migration
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F25%3A00637392" target="_blank" >RIV/68378050:_____/25:00637392 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1126/sciadv.adu6361" target="_blank" >https://doi.org/10.1126/sciadv.adu6361</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1126/sciadv.adu6361" target="_blank" >10.1126/sciadv.adu6361</a>
Alternative languages
Result language
angličtina
Original language name
Live-cell magnetic manipulation of recycling endosomes reveals their direct effect on actin protrusions to promote invasive migration
Original language description
Endocytic recycling pathways play key roles in a broad range of cellular processes, and many vesicle trafficking regulators are implicated in progression of disease such as cancer. The Rab11 family (Rab11a, Rab11b, and Rab25) controls the return of internalized cargos to the plasma membrane, and Rab25 has been implicated in the aggressiveness of cancer by promoting invasive migration. However, while Rab25 vesicles distribute to the leading edge of moving cells, how directly they contribute to cell protrusion is not clear. Here, we adopt a magnetogenetic approach that allows direct manipulation of Rab25 positioning to show that localization to the cell periphery drives the formation of F-actin protrusions. We demonstrate that endogenous Rab25 vesicles coordinate the positioning of key cargos, including the actin regulator FMNL1 and integrin beta 1, with the activation of Rho guanosine triphosphatases at the plasma membrane to generate and maintain F-actin-rich filopodium-like protrusions and promote cancer cell invasive migration in the three-dimensional matrix.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Science Advances
ISSN
2375-2548
e-ISSN
2375-2548
Volume of the periodical
11
Issue of the periodical within the volume
27
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
eadu6361
UT code for WoS article
001522918900001
EID of the result in the Scopus database
2-s2.0-105010547581