Live-cell imaging of mammary organoids using light sheet microscopy

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F25%3A00638799" target="_blank" >RIV/68378050:_____/25:00638799 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/25:00141988
Result on the web
<a href="https://doi.org/10.1007/s10911-025-09587-3" target="_blank" >https://doi.org/10.1007/s10911-025-09587-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10911-025-09587-3" target="_blank" >10.1007/s10911-025-09587-3</a>

Result language
angličtina
Original language name
Live-cell imaging of mammary organoids using light sheet microscopy
Original language description
The mammary gland is a dynamic organ whose parenchyma undergoes major development during puberty and extensive remodeling with each estrous cycle. These processes can be modelled and investigated in vitro via 3D cell culture techniques that employ specialized extracellular matrices and appropriate growth factors. The resulting mammary organoid cultures faithfully represent the mammary gland with respect to cellular heterogeneity, cell-cell contacts, overall architecture as well as response to growth factor stimuli and are amendable to a variety of molecular methods as well as microscopy techniques. Among the imaging techniques, light sheet microscopy (single plane illumination microscopy, SPIM) represents a useful method for longitudinal monitoring of morphological changes and cell behavior during the establishment of mammary gland ductal systems. In contrast to other fluorescence microscopy techniques such as widefield- and confocal-microscopy, SPIM exerts minimal phototoxicity while allowing fast acquisition of different fluorophores within organoids arranged in a 3D matrix under optimized environmental conditions. Here, we provide a detailed protocol for organoid acquisition and culture and describe two sample mounting variants for use with multiview and inverted light sheet microscopes.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10601 - Cell biology

Project
<a href="/en/project/LL2323" target="_blank" >LL2323: Deciphering the role of fibroblast mechanical forces in epithelial morphogenesis</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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