Contractile fibroblasts form a transient niche for the branching mammary epithelium

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F25%3A00640287" target="_blank" >RIV/68378050:_____/25:00640287 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/25:00142477
Result on the web
<a href="https://doi.org/10.1038/s41467-025-63612-x" target="_blank" >https://doi.org/10.1038/s41467-025-63612-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41467-025-63612-x" target="_blank" >10.1038/s41467-025-63612-x</a>

Result language
angličtina
Original language name
Contractile fibroblasts form a transient niche for the branching mammary epithelium
Original language description
Fibroblasts are stromal cells found in connective tissue that are critical for organ development, tissue homeostasis and pathology. Single-cell transcriptomic analyses have revealed a high level of inter- and intra-organ heterogeneity of fibroblasts. However, the functional implications and lineage relations of different fibroblast subtypes remained unexplored, especially in the mammary gland. Here, we provide a comprehensive characterization of pubertal mouse mammary fibroblasts, through single-cell RNA sequencing, spatial mapping, functional assays, and in vivo lineage tracing. We unravel a transient niche-forming population of specialized contractile fibroblasts that exclusively localize around the tips of the growing mammary epithelium and are recruited from preadipocytes in the surrounding fat pad stroma. Using organoid-fibroblast co-cultures we reveal that different fibroblast populations can acquire contractile features when in direct contact with the epithelium, promoting organoid branching. The detailed in vivo characterization of these specialized cells and their lineage history provides insights into fibroblast heterogeneity and implicates their importance for creating a signalling niche during mouse mammary gland development.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10601 - Cell biology

Project
<a href="/en/project/GA23-04974S" target="_blank" >GA23-04974S: Coupled chemo-mechanical model of normal and cancer microenvironment</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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