Establishing standardized transthoracic echocardiography reference ranges for mouse models: insights into the impact of anesthesia, sex, and age

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F25%3A00644523" target="_blank" >RIV/68378050:_____/25:00644523 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.3389/fcvm.2025.1695034" target="_blank" >https://doi.org/10.3389/fcvm.2025.1695034</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fcvm.2025.1695034" target="_blank" >10.3389/fcvm.2025.1695034</a>

Result language

angličtina

Original language name

Establishing standardized transthoracic echocardiography reference ranges for mouse models: insights into the impact of anesthesia, sex, and age

Original language description

Introduction Mouse models play a critical role in cardiology research, offering valuable insights into the molecular mechanisms, genetics, and potential treatments for cardiovascular diseases. However, the ability to transfer findings in mice between studies is limited by the absence of standardized protocols and valid reference values for the assessment of normal cardiac function in mice. This study aims to establish comprehensive transthoracic echocardiography (TTE) reference ranges for mice, particularly focusing on C57BL/6N wild-type controls.Methods The study, which includes data from over 15,000 mice through the International Mouse Phenotyping Consortium (IMPC), highlights how variables such as sex, age, body weight, and anesthesia impact TTE parameters.Results The findings showed that anesthesia is the primary predictor of variability in cardiac function. Isoflurane- and tribromoethanol-anesthetized mice presented with modified cardiac function compared with conscious mice. In addition, we observed minimal sex differences in cardiac morphology and function, except for small variations influenced by anesthesia. The effects of aging on cardiac function were modest, characterized by a decrease in heart rate and subtle changes in ventricular dimensions without evidence of pathological remodeling, possibly attributable to disease-free cardiovascular aging.Discussion Validation of the reference ranges across multiple mouse strains showed that these values provide a reliable baseline for experiments involving cardiac function in mice. The data underscore the importance of using anesthesia-specific reference values when interpreting TTE results, ensuring robust comparisons in genetic and pharmacological studies. These reference ranges serve as quality assurance tools for future cardiac studies in mice, offering insights into typical TTE parameter values, supporting the detection of experimental perturbations, and contributing to more effective translation of findings from mice to humans.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Cardiovascular Medicine

ISSN

2297-055X

e-ISSN

2297-055X

Volume of the periodical

12

Issue of the periodical within the volume

19 Dec

Country of publishing house

CH - SWITZERLAND

Number of pages

19

Pages from-to

1695034

UT code for WoS article

001652184600001

EID of the result in the Scopus database

2-s2.0-105026501645