Damage to dry plasmid DNA induced by nanosecond XUV-laser pulses
Result description
We have studied the nature of DNA damage induced directly by the pulsed 46.9 nm radiation provided by a capillary-discharge Ne-like Ar laser (CDL). Different surface doses were delivered with a repetition rate of a few Hz and an average pulse energy similar to 1 mu J. A simple model DNA molecule, i.e., dried closed-circular plasmid DNA (pBR322), was irradiated. The agarose gel electrophoresis method was used for determination of both SSB and DSB yields.
Keywords
extreme ultraviolet laserradiation damage to DNAsingle-strand breaksdouble-strand breakspulsed laser deposition
The result's identifiers
Result code in IS VaVaI
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Damage to dry plasmid DNA induced by nanosecond XUV-laser pulses
Original language description
We have studied the nature of DNA damage induced directly by the pulsed 46.9 nm radiation provided by a capillary-discharge Ne-like Ar laser (CDL). Different surface doses were delivered with a repetition rate of a few Hz and an average pulse energy similar to 1 mu J. A simple model DNA molecule, i.e., dried closed-circular plasmid DNA (pBR322), was irradiated. The agarose gel electrophoresis method was used for determination of both SSB and DSB yields.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
BH - Optics, masers and lasers
OECD FORD branch
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Result continuities
Project
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Damage to VUV, EUV, and X-ray Optics III
ISBN
9780819486677
ISSN
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e-ISSN
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Number of pages
8
Pages from-to
"80770W/1"-"80770W/8"
Publisher name
SPIE
Place of publication
Bellingham
Event location
Prague
Event date
Apr 18, 2011
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000293212000021
Basic information
Result type
D - Article in proceedings
CEP
BH - Optics, masers and lasers
Year of implementation
2011