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Breaking DNA strands by extreme-ultraviolet laser pulses in vacuum

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F15%3A00448547" target="_blank" >RIV/68378271:_____/15:00448547 - isvavai.cz</a>

  • Alternative codes found

    RIV/61389005:_____/15:00448547 RIV/68407700:21340/15:00235107

  • Result on the web

    <a href="http://dx.doi.org/10.1103/PhysRevE.91.042718" target="_blank" >http://dx.doi.org/10.1103/PhysRevE.91.042718</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1103/PhysRevE.91.042718" target="_blank" >10.1103/PhysRevE.91.042718</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Breaking DNA strands by extreme-ultraviolet laser pulses in vacuum

  • Original language description

    Ionizing radiation induces a variety of DNA damages including single-strand breaks (SSBs), double-strand breaks (DSBs), abasic sites, modified sugars, and bases. We have studied the nature of DNA damage induced directly by the pulsed 46.9nm (26.5 eV) radiation provided by an extreme ultraviolet (XUV) capillary-discharge Ne-like Ar laser (CDL). Doses up to 45 kGy were delivered with a repetition rate of 3 Hz. We studied the dependence of the yield of SSBs and DSBs of a simple model of DNA molecule (pBR322) on the CDL pulse fluence. Agarose gel electrophoresis method was used for determination of both SSB and DSB yields. The action cross sections of the single and double-strand breaks of pBR322 plasmid DNA in solid state were determined. We observed an increase in the efficiency of strand-break induction in the supercoiled DNA as a function of laser pulse fluence. Results are compared to those acquired at synchrotron radiation facilities and other sources of XUV and soft x-ray radiation.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    BO - Biophysics

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Physical Review. E

  • ISSN

    1539-3755

  • e-ISSN

  • Volume of the periodical

    91

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

    "042718-1"-"042718-8"

  • UT code for WoS article

    000353637500008

  • EID of the result in the Scopus database

    2-s2.0-84929192771