Dose-Rate Effects in Breaking DNA Strands by Short Pulses of Extreme Ultraviolet Radiation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F18%3A00506556" target="_blank" >RIV/61388955:_____/18:00506556 - isvavai.cz</a>
Alternative codes found
RIV/68378271:_____/18:00496971 RIV/61389021:_____/18:00503532 RIV/61389005:_____/18:00496971 RIV/68407700:21340/18:00324188
Result on the web
<a href="http://hdl.handle.net/11104/0297789" target="_blank" >http://hdl.handle.net/11104/0297789</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1667/RR14825.1" target="_blank" >10.1667/RR14825.1</a>
Alternative languages
Result language
angličtina
Original language name
Dose-Rate Effects in Breaking DNA Strands by Short Pulses of Extreme Ultraviolet Radiation
Original language description
In this study, we examined dose-rate effects on strand break formation in plasmid DNA induced by pulsed extreme ultraviolet (XUV) radiation. Dose delivered to the target molecule was controlled by attenuating the incident photon flux using aluminum filters as well as by changing the DNA/buffer-salt ratio in the irradiated sample. Irradiated samples were examined using agarose gel electrophoresis. Yields of single-and double-strand breaks (SSBs and DSBs) were determined as a function of the incident photon fluence. In addition, electrophoresis also revealed DNA cross-linking. Damaged DNA was inspected by means of atomic force microscopy (AFM). Both SSB and DSB yields decreased with dose rate increase. Quantum yields of SSBs at the highest photon fluence were comparable to yields of DSBs found after synchrotron irradiation. The average SSB/DSB ratio decreased only slightly at elevated dose rates. In conclusion, complex and/or clustered damages other than cross-links do not appear to be induced under the radiation conditions applied in this study. (C) 2018 by Radiation Research Society.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Radiation Research
ISSN
0033-7587
e-ISSN
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Volume of the periodical
189
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
466-476
UT code for WoS article
000430572800003
EID of the result in the Scopus database
2-s2.0-85045934898