DNA strand breaks induced by soft X-ray pulses from a compact laser plasma source

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F16%3A00464879" target="_blank" >RIV/68378271:_____/16:00464879 - isvavai.cz</a>
Alternative codes found
RIV/61389005:_____/16:00464879 RIV/68407700:21340/16:00310750
Result on the web
<a href="http://dx.doi.org/10.1016/j.radphyschem.2015.11.021" target="_blank" >http://dx.doi.org/10.1016/j.radphyschem.2015.11.021</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.radphyschem.2015.11.021" target="_blank" >10.1016/j.radphyschem.2015.11.021</a>

Result language
angličtina
Original language name
DNA strand breaks induced by soft X-ray pulses from a compact laser plasma source
Original language description
Application of a compact laser plasma source of soft X-rays in radiobiology studies is demonstrated. The source is based on a laser produced plasma as a result of irradiation of a double-stream gas puff target with nanosecond laser pulses from a commercially available Nd:YAG laser. The source allows irradiation of samples with soft X-ray pulses in the "water window" spectral range (wavelength: 2.3-4.4 nm; photon energy: 280-560 eV) in vacuum or a helium atmosphere at very high-dose rates and doses exceeding the kGy level. Single-strand breaks (SSB) and double-strand breaks (DBS) induced in DNA plasmids pBR322 and pUC19 have been measured. The different conformations of the plasmid DNA were separated by agarose gel electrophoresis. An exponential decrease in the supercoiled form with an increase in linear and relaxed forms of the plasmids has been observed as a function of increasing photon fluence. Significant difference between SSB and DSB in case of wet and dry samples was observed that is connected with the production of free radicals in the wet sample by soft X-ray photons and subsequent affecting the plasmid DNA. Therefore, the new source was validated to be useful for radiobiology experiments.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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